About MEDIA CHEMICALS

A. MICROBIOLOGY CULTURE MEDIA

1. Nutrient Media (General Purpose)

1. Nutrient Agar (NA)

Application:

• Growth of non-fastidious bacteria.

• Routine cultivation and maintenance.

2. Nutrient Broth (NB)

Application:

• Liquid growth of bacteria.

• Preparation of inocula.

2. Selective Media

3. MacConkey Agar

Application:

• Selective for Gram-negative bacteria.

• Differentiates lactose fermenters (pink) from non-fermenters.

4. Mannitol Salt Agar (MSA)

Application:

• Selective for Staphylococcus species.

• Differentiates S. aureus (yellow zones).

5. Sabouraud Dextrose Agar (SDA)

Application:

• Selective for fungi & yeasts.

3. Differential Media

6. Blood Agar

Application:

• Differentiates bacteria by hemolysis (, , ).

• Grows fastidious organisms.

7. Eosin Methylene Blue Agar (EMB)

Application:

• Differentiates coliforms.

• E. coli shows metallic green sheen.

4. Enriched Media

8. Chocolate Agar

Application:

• Grows fastidious organisms like Neisseria and Haemophilus.

9. Brain Heart Infusion (BHI) Agar/Broth

Application:

• Culturing bacteria, yeasts, and molds.

• Used in clinical laboratories.

5. Anaerobic Media

10. Thioglycollate Broth

Application:

• Supports growth of aerobic and anaerobic bacteria depending on oxygen gradient.

11. Robertsons Cooked Meat Medium

Application:

• Isolation of anaerobes, especially Clostridium species.

B. CELL CULTURE MEDIA (Animal Cell Culture)

12. DMEM (Dulbeccos Modified Eagle Medium)

Application:

• Widely used for mammalian cells (HEK293, fibroblasts, etc.).

13. RPMI-1640

Application:

• Used for lymphocytes, hybridoma cells, and hematopoietic cells.

14. MEM (Minimum Essential Medium)

Application:

• Cultures a variety of adherent cell lines.

15. F-12 (Hams F-12)

Application:

• Used for CHO cells, cancer cells, and co-cultures.

16. DMEM/F12 (Mixed medium)

Application:

• Used for mixed cell types and primary cells.

C. PLANT TISSUE-CULTURE MEDIA

17. MS Media (Murashige & Skoog)

Application:

• Plant tissue culture, shoot regeneration, callus induction.

18. B5 Media (Gamborg B5)

Application:

• Cell suspensions, callus cultures.

19. Whites Medium

Application:

• Root culture studies.

D. MICROBIOLOGY SPECIALTY MEDIA

20. XLD Agar (Xylose Lysine Deoxycholate)

Application:

• Selective for Salmonella & Shigella.

21. TCBS Agar

Application:

• Isolation of Vibrio species.

22. Cetrimide Agar

Application:

• Selective for Pseudomonas aeruginosa.

23. LowensteinJensen (LJ) Medium

Application:

• Culturing Mycobacterium tuberculosis.